QUANTITATIVE HISTOLOGY

Materials in this section were created and kindly offered by Zbyněk Tonar, M.D., M.Sc., Ph.D. and his co-workers from the Dept. of Histology and Embryology, Medical Faculty in Pilsen, Charles University, Czech Republic.

It contains "Atlas of Quantitative histology" (in PDF format) and following set of animations demonstrating the use of Ellipse to solve some basic problems of quantitative histology. Animations are in *.swf format and require proper player (Adobe Flash Player).

• Calibration of image in X, Y, Z directions
• Estimation of area and volume by using point test grid (artery)
  
• Estimation of area and volume by using point test grid in immunohistochemistry
  
• Estimation of length and length density by using cycloids
  
• Estimation of surface area and surface density by a cycloid system
  
• Estimation of the numerical density of objects by optical disector applied to optical serial sections
  
• Estimation of the numerical density of objects by physical disector applied to aligned serial sections
  
• Estimation of the number of objects profiles per unit area of a section
  
• Systematic uniform random selection of counting frames in high resolution image
  
• Delaunay triangulation of 2-D points mesh
  
• Cluster analysis in 2-D
  
• Orientation of structures in 2-D
  
• Length estimation in 3-D and orientation of structures in 3-D
  
• 3-D reconstruction of object contours
  
• 3-D reconstruction of object surface